Voiced by: Tasia Valenza
Classification: Gadget Character (without explosives)
Infused with plant based powers and the ability to control plant-life, Pamela Isley has begun to identify more with plants than with humans. As Poison Ivy, she terrorizes Gotham City with her plans to wipe out humanity.
Intro: The camera shows a large concentration of plant life, covered in leaves and bushes. It soon moves out of the way on its own, with Poison Ivy stepping out and readying to fight.
Post-Round Taunt: Poison Ivy grows flowers around her, either laughing or saying "Now to finish you off."
Outro: Ivy says "Nice try, idiot." and lifts her arm up, causing vines to sprout from the ground and grab the opponent, lifting them up behind her. As they struggle in the background, Ivy creates a throne of plant-life and sits down, looking at the camera.
* Underfoot Sprout - Creates a large venus fly trap plant underneath where the opponent is standing
** Meter Burn - Instead of a venus fly trap, Ivy creates vines that hold the opponent in place
* Blow Kiss - Poison Ivy blows a kiss that sends forward spores, temporarily stunning the opponent
** Meter Burn - Poison Ivy blows a kiss full of mind control spores, which not only damage the opponent, but makes them walk towards Ivy (like one of Quan Chi's moves in MK9)
* Vine Whip - Causes a vine to spurt from the ground and swat towards the opponent
** Meter Burn - Causes multiple vines to spurt from the ground, hitting the opponent one after another
* Spore Shot - Ivy summons a plant that shoots a large, glowing, poisonous spore in the direction of the opponent, similar to the ones seen in Batman: Arkham Asylum
** Meter Burn - The plant that Ivy summons instead shoots three spores in rapid succession
* Spore Leech - Poison Ivy creates a plant that shoots forward a gas that, if it connects with the opponent, steals some of their health and adds it to Poison Ivy's health bar
** Meter Burn - When enhanced, the move takes more health and adds it to Ivy's
* Rose's Thorn - Creates a thorny vine that spins around her, damaging opponents and blocking projectiles
** Meter Burn - The vine spins around her, and if an opponent touches the vines, they will be ensnared
Launcher: Holds her hand forward and makes a large plant sprout from the ground and ram into the opponent
Forward - Entangles the opponent in vines, seductively rubs their face, then has the vines throw the opponent forward
Back - Entangles the opponent in vines, seductively walks around them, then has the vines throw the opponent backward
Poison Spores - Poison Ivy lets off pink spores from her body that will poison the opponent if they touch her, slowly draining their health. Once used, it takes a few seconds to recharge. If she gets hit while using the spores, the spores will disappear.
Nature's Wrath - Poison Ivy sends out vines from the floor, and if they grab the opponent, she will have them throw the opponent into the mouth of a newly created, giant venus fly trap. The camera shows the opponent being burn in the plant's digestive juices, before they spill out back onto the stage.
Ending: Having previously been a prisoner of Superman's Regime, Poison Ivy had freed herself and gotten her revenge. With Superman gone and the world without structure, Ivy saw her chance to enact her more sinister plans. Using her control over plant life, Poison Ivy had her "babies" hunt down every last human being on Earth. With humanity extinct, Poison Ivy had finally created a natural paradise for all plant life to thrive.
"You'll make nice mulch."
"My babies will strangle you."
"Lay down and die!"
"Feel the wrath of Mother Nature!"
Poison Ivy - "Show me what you've got, kid."
Nightwing - "You first."
Poison Ivy - "You don't stand a chance, Batman!"
Batman - "That's what you said last time."
Poison Ivy - "Just accept nature's will."
Batman - "I don't need to. I'm Batman."
Poison Ivy - "I'll make you my slave!"
Batman - "I'd never give in to you."
Poison Ivy - "You'll pay for hurting Harley!"
Joker - "Alright, I'll cut you a check."
Poison Ivy - "I'll enjoy killing you."
Joker - "Time to do some weeding."
Poison Ivy - "Leave him behind, Harley!"
Harley Quinn - "I'm not leavin' my pud'n!"
Poison Ivy - "You're the only human I could tolerate."
Harley Quinn - "Gee, thanks, Red!"
Poison Ivy - "Sorry about this, Harley."
Harley Quinn - "I thought I was on your 'do not kill' list!"
Poison Ivy - "So, you're connected to the Green."
Solomon Grundy - "Grundy not green. You green!"
Poison Ivy - "I'll have to kill you, Selina."
Catwoman - "You'll have to try."
Poison Ivy - "Going to water some plants?"
Aquaman - "You're just hilarious."
Nightwing - "Where'd I leave my weedwhacker?"
Poison Ivy - "You monster!"
Nightwing - "Are you trying to seduce me?"
Poison Ivy - "Depends. Is it working?"
Batman - "It's over, Isley."
Poison Ivy - "Maybe for you."
Batman - "I'm sending you back to Arkham."
Poison Ivy - "I'll only escape again."
Batman - "Your 'babies' won't stop me."
Poison Ivy - "I'll feed you to them!"
Batman - "You're barely human anymore."
Poison Ivy - "That's a good thing."
Joker - "You and Harley are friends. Think we could--?"
Poison Ivy - "Not in your wildest dreams, clown."
Joker - "How about some nice Joker Toxin?"
Poison Ivy - "Pff, you call that stuff poison?"
Harley Quinn - "Show me what you got, Red!"
Poison Ivy - "Whatever you say, Harley."
Harley Quinn - "Did I tell ya you look nice today?"
Poison Ivy - "Well, this pretty flower has thorns!"
Harley Quinn - "Don't lecture me about Mr. J!"
Poison Ivy - "He's not good for you, Harley!"
Solomon Grundy - "Grundy crush plant lady!"
Poison Ivy - "Oh, you like to play rough?"
Catwoman - "Jeez, calm down, Ivy."
Poison Ivy - "I'll calm down when you're dead!"
* Tasia Valenza previously voiced Poison Ivy in the Batman Arkham games
* In the Arkham Asylum/Joker's Asylum stage, instead of stomping on the ground to activate the flower interactable like other gadget characters, Ivy would move her arms like she's controlling the flower to make it shoot its poison gas
* Poison Ivy is a ranged character, as her moveset does not have much to offer for close-up confrontation